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BET9登录网址开发了两种差别类型的Anti-AAV抗体
。
第一种Anti-AAV抗体可识别特定AAV病毒颗粒构象表位，适用于Dot blot快速检测病毒载体表达水平和Sandwich ELISA定量剖析AAV物理滴度
。

我们提供经由预验证的即用型抗体对，利便您的研究
。

第二种Anti-AAV抗体可识别三种 AAV 衣壳卵白 VP1、VP2 和 VP3 的线性表位
。这类抗体适用于Western Blot剖析，可用于评估 AAV 生产中 VP1/VP2/VP3 的表达水平，或判断 AAV 生产和纯化历程中衣壳卵白的保存
。

产品应用

• 衣壳卵白VP1, VP2, VP3判断

  AAV 衣壳由三种衣壳卵白VP1、VP2 和 VP3 以 1:1:10 的比例总共 60 个拷贝组成
  。 VP1、VP2、VP3的表观分子量划分为87kDa、73kDa、62kDa
  。衣壳卵白对细胞转导效率至关主要
  。 VP2和VP3缺乏被以为是无效包装的主要缘故原由
  。过量的 VP3 可能会降低转导效率
  。三种衣壳卵白比例的优化在 AAV 生产历程中至关主要
  。聚丙烯酰胺凝胶电泳 (PAGE) 和卵白质印迹 (WB) 是在重组 AAV 生产历程的初始优化和质量控制历程中检测 VP1、VP2 和 VP3 卵白组成的有用要领
  。 GenScript 提供Anti-AAV 抗体用于表征衣壳卵白 VP1、VP2 和 VP3（图 1 和 2）
  。

  

  Figure 1. VP1, VP2, VP3 protein Analysis of AAV Capsids by Western blot
  
  

  Western blot analysis of VP1, VP2, VP3 proteins of AAV type 1, 2, 5, 6, 8, 9, DJ and rh.1 was conducted using MonoRab? AAVX VP1/VP2/VP3 Antibody (5G4), mAb, Rabbit (GenScript, A02206, 0.1 ?g/mL). The signal was developed with Peroxidase Conjugated Goat Anti-Rabbit IgG.
  
  

  Predicted Size for VP1, VP2 and VP3: 87, 72 and 62 kDa
  Observed Size for VP1, VP2 and VP3: 87, 72 and 62 kDa

  

  Figure 2. VP1 protein Analysis of AAV Capsids by Western blot
  
  

  Western blot analysis of VP1 protein of AAV type 1, 2, 5, 6, 8, 9, DJ and rh.1 was conducted using MonoRab? AAVX VP1 Antibody (24F5), mAb, Rabbit (GenScript, A02207, 0.1 ?g/mL). The signal was developed with Peroxidase Conjugated Goat Anti-Rabbit IgG.
  
  

  Predicted Size: 87 kDa
  Observed Size: 87 kDa

• 物理病毒滴度定量检测

  三质粒转染HEK293细胞系统是生产rAAV的常用要领
  。病毒颗粒滴度（物理滴度）的检测在rAAV生产历程中很主要，下图展示了用Anti-AAV抗体定量检测AAV滴度的原理（图3）
  。BET9登录网址提供多种Anti-AAV 抗体用于 AAV 物理滴度的定量（图 4 和 5）
  。

  

  Figure 3. Principle of AAV titer quantitation with AAV antibody

  

  Figure 4. Standard Curve of AAV2 Capsids by Sandwich ELISA
  
  

  The Sandwich ELISA assay was developed by Anti-AAV2 (intact particle) Antibody (2C4), mAb, Mouse (GenScript, A02159-40; 2 μg/mL) and Anti-AAV2 (intact particle) Antibody (2C4) [Biotin], mAb, Mouse (GenScript, A02160-40; 2 μg/mL) as the capture and detection antibody, respectively.

  

  Figure 5. Standard curve of AAV8 by Sandwich ELISA
  
  

  The AAV8 Sandwich ELISA assay was developed by using MonoRab? AAV8 (intact particle) Antibody (75F2), mAb, Rabbit (GenScript, A02204; 2.5 μg/mL) and MonoRab? AAV8 (intact particle) Antibody (72E8), mAb, Rabbit (GenScript, A02205; 1 μg/mL) as the capture and detection antibody, respectively.

• 免疫原性剖析

  人体内的抗AAV抗体可经由野生型AAV病毒熏染爆发，这些预先保存的抗AAV抗体可能会给基因治疗带来潜在的清静危害，因此有须要彻底地表征rAAV 产品免疫原性
  ？挂┪锟固 (ADA) 检测是丈量预先保存的抗AAV 抗体的要害工具，图 6 形貌了一种抗 AAV 抗体检测的形式
  。BET9登录网址的Anti-AAV 抗体可用作免疫原性剖析的阳性比照或在 ADA 剖析中制备标准曲线（图 7 和 8）
  。

  

  Figure 6. Anti-AAV antibody assay format

  

  Figure 7. Standard Curve of AAV2 Capsids by Indirect ELISA
  
  

  ELISA binding of MonoRab? AAV2 (intact particle) Antibody (33G5), mAb, Rabbit (GenScript, A02203) with AAV2.
  Coating antigen: AAV2, 1 ?g/mL.
  MonoRab? AAV2 (intact particle) Antibody (33G5), mAb, Rabbit (GenScript, A02203) dilution start from 1,000 ng/mL.

  

  Figure 8. Standard Curve of AAV8 Capsids by Indirect ELISA
  
  

  ELISA binding of MonoRab? AAV8 (intact particle) Antibody (75F2), mAb, Rabbit (GenScript, A02204) with AAV type 8.
  Coating antigen: AAV type 8, 1 ?g/mL.
  MonoRab? AAV8 (intact particle) Antibody (75F2), mAb, Rabbit (GenScript, A02204) dilution start from 1,000 ng/mL.

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